reverse transcription

Functional analysis based on these structures also revealed how a "prime editor" could achieve reverse transcription, synthesizing DNA from RNA, without "cutting" both strands of the double helix.

Finally overcoming the challenges, the researchers succeeded in determining the three-dimensional structure of the prime editor complex in multiple states during reverse transcription on the target DNA.

While performing the reverse transcription, the reverse transcriptase maintained its position relative to the Cas9 protein.

“Under normal circumstances, there is so little reverse transcription machinery available” in human cells, Malik says.

In both cases, labs analyze the samples using quantitative reverse transcription polymerase chain reaction tests, which can detect genetic material from SARS-CoV-2 and quantify the number of viral particles in each milliliter of sample.