N terminus

The N terminus of Dot1 is predicted to be an IDR and can promote H2B ubiquitination when overexpressed19.

The numberings of nearby amino acid residues, which are shown along with these cofactors, are listed below each cofactor. b, Fitting of different secondary structure elements to cryo-EM density. c, Eleven identified lipids are modelled as phosphatidylethanolamine molecules. d, The triacylated cysteine at the N terminus of ActE shown along with 15 downstream amino acids.

In the Supplementary Information of this Article, on pages 3, 8 and 11, we refer to “UK Patent Application No. 1522431.4”, which pertains to the discovery of the truncated peptide analogues we used1, and specifically to the use of the modified N terminus or ‘Cap’.

This helix is also mentioned in the main text, where “positioned near the N terminus of helix αA in CD” should have read “positioned near the N terminus of helix αE in CD”.

Hence, ABL001 binding mimics the structural consequences of myristate binding to the N terminus of ABL1.